RT-PCR made EASY – Reverese Transcriptase PCR for detection and test of Viral DNA
RT-PCR – Reverese Transcriptase PCR for detection of Viral DNA
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In order for a virus like the coronavirus to be detected by a PCR, scientists need to convert the RNA to DNA. This is a process called ‘reverse transcription’. If you remember the process of copying of DNA to RNA was known as transcritption, hence the process of using rna to form dna is known as revese transcritption.
Hence using this technique of reverse transcription with the technique of Polymerase chain reaction is known as RT-PCR.
An enzyme is used by the scientists which converts the RNA into DNA and the PCR makes millions of copies of this viral DNA in no time. And subsequently scientists have a large enough quantity of viral DNA to accurately confirm that the virus is present in the sample or not.